physarum polycephalum temperature

1976 Aug 2;146(3):313-5 J Bacteriol. Molecular and General Genetics MGG, Vol. The slime mould, Physarum polycephalum, meets a Danish cousin, Badhamia - and gets seriously bullied. © 1939 Wiley The plasmodium of Physarum polycephalum is a unicellular and multinuclear giant amoeba that shows adaptive behaviors. The latter shows a striking reduction in the frequency of postmitotic reconstruction nuclei at the restrictive temperature. 1, p. movement of Physarum polycephalum M Iima et al Studies of the phase gradient at the boundary of the phase diffusion equation, motivated by peculiar wave patterns of rhythmic contraction in the amoeboid movement of Printed in The interrelationships of the factors of temperature and pH upon the fruiting of Physarum polycephalum Schw. JSTOR®, the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks of ITHAKA. Isolation of cell cycle mutants of Physarum polycephalum. COVID-19 is an emerging, rapidly evolving situation. Under the conditions of the above experiments, sclerotia cannot be produced at high temperatures (32.5⚬-35.0⚬) nor at low temperatures (8⚬-12⚬). NLM -, Toxicol Appl Pharmacol. Complementation in heterokaryons was used to sort the mutants into nine functional groups. Published by the Botanical Society of America continuously since 1914, the American Journal of Botany (AJB) is the Society’s flagship research journal. The thermotaxis of the plasmodium is so strong and the organism without learning always proceeds to the higher temperature region. Generally speaking, the necessity of light for fruiting cannot be dispensed with by increasing acidity nor by lowering the temperature. Select the purchase National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. 1976 Nov 24;149(1):115-9. doi: 10.1007/BF00275966. Physarum polycephalum belongs to the Amoebozoa, the sister group to the Opisthokonts (i.e., fungi and animals) ( Cavalier-Smith 2003) which both together form the supergroup Amorphea ( … NIH The petri dishes were wrapped in aluminum foil at room temperature (22-25°C) and sealed with parafilm. Cycloheximide resistance of Physarum polycephalum. Eur J Biochem. Our core businesses produce scientific, technical, medical, and scholarly journals, reference works, books, database services, and advertising; professional books, subscription products, certification and training services and online applications; and education content and services including integrated online teaching and learning resources for undergraduate and graduate students and lifelong learners. JSTOR is part of ITHAKA, a not-for-profit organization helping the academic community use digital technologies to preserve the scholarly record and to advance research and teaching in sustainable ways. Curr Genet. Access supplemental materials and multimedia. When transferred to the restrictive temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing. Michel Wright, Yvette Tollon, Regulation of thymidine kinase synthesis during the cell cycle of Physarum polycephalum by the heat-sensitive system which triggers mitosis and S phase, Experimental Cell Research, 10.1016/0014-4827(79)90304-5, 122, 2, (273-279), (1979). Would you like email updates of new search results? 163, Issue. Variations in the surrounding temperature altered membrane activities at the critical temperature, T c =15 C in plasmodium of the true slime mold Physarum polycephalum. de Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the West of England, Bristol, UK. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. Regulation of protein synthesis in the plasmodial phase of Physarum polycephalum.  |  Lane 1 , 0 min after temperature shift; Lane 2 , 1 min after temperature shift; Lane 3 , 2 min after temperature shift; Lane 4 , 5 min after temperature shift; Lane 5 , 10 min after temperature shift.  |  Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature. Wiley has partnerships with many of the world’s leading societies and publishes over 1,500 peer-reviewed journals and 1,500+ new books annually in print and online, as well as databases, major reference works and laboratory protocols in STMS subjects. AB259870). Whiting 1, Ben P.J. Physarum polycephalum (Slime mold) Status Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity i Milk clotting activity. Photobiol. In the absence of oxygen, no negative II. 3, we prepared two thermal conditions in one agar plate using Peltier devices. Phototaxis in Physarum polycephalum 271 A tungsten 8V-30W microscope lamp (Olympus Co., Tokyo) was used to observe proto-plasmic … On the day before the measurement, dried P. polycephalumwas placed on an agarose gel and lightly sprayed with water to induce germination. 1981 Dec;120(3):529-34. doi: 10.1111/j.1432-1033.1981.tb05732.x. J Comput Theore Nanosci 8(3): 383-390. Theory of time-resolved somatic complementation and its use to explore the sporulation control network in Physarum polycephalum. Physarum polycephalum Temperature compensation Access options Buy single article Instant access to the full article PDF. The higher the temperature at which a plasmodium is maintained, the greater the acidity must be in order to produce fruiting. Wiley has published the works of more than 450 Nobel laureates in all categories: Literature, Economics, Physiology or Medicine, Physics, Chemistry, and Peace. 1978 Mar;43(3):449-59 Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication. -, Cell. Of the two lytic mutants, one affected both amoebae and plasmodia, and the other affected plasmodia alone. AJB publishes peer-reviewed, innovative, significant research of interest to a wide audience of scientists in all areas of plant biology (e.g., biodiversity, structure, function, development, genetics, evolution, reproduction, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). This site needs JavaScript to work properly. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in … Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvation. option. -. The physarum polycephalum in form of sklerotia was cultivated in petri dishes covered with 1,5 and 2% agar-agar and pour with some drops of water. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. In the temperature range around 10–30 °C, the plasmodium shows thermotaxis to the higher temperature. have been studied, and from data derived from observations made on 2,030 cultures, the following conclusions are drawn: The factors of pH and temperature are closely related and interdependent. • Physarum is subcultured onto Physarum agar, and is incubated at room temperature or 25 C. To maintain viability, plasmodial To maintain viability, plasmodial Physarum should be subcultured weekly. 1980;179(1):43-8. doi: 10.1007/BF00268444. Changes in the membrane and zeta potentials, which were induced by chemical stimuli, decreased when the temperature decreased below T c . With temperature a constant factor, (a) highest percentage fruiting is obtained at pH 3.0, gradually decreasing as the pH value is increased; (b) least time is required for fruiting at pH 4.0. The growth-defective mutants were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior. -, Genet Res. 163, Issue. 1975 Jun;62(6):283-9. doi: 10.1007/BF00608954. Maximum temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C. 1, p. 91. The negative selectant, netropsin, was used to enrich for temperature-sensitive mutants among a population of mutagenized amoebae, and, separately, a nonselective screening method was used to isolate plasmodial temperature-sensitive mutants among clonal plasmodia derived from mutagenized amoebae. As stated before, pH values at this temperature 1977 Aug;131(2):473-6 Thin layer chromatogram of crude lipids from Physarum cells at various periods after temperature shift from 24 C to 40 C. Lipids in each lane were extracted from 6.4 × 10 6 cells. The P. polycephalumcultured on the gel vividly about 12 hours later. Check out using a credit card or bank account with. Molecular and General Genetics MGG, Vol. [Modern research on the cell cycle and mitosis in the myxomycete Physarum polycephalum]. Execution of this step is necessary for both mitosis and chromosome replication. J Bacteriol. Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Founded in 1807, John Wiley & Sons, Inc. has been a valued source of information and understanding for more than 200 years, helping people around the world meet their needs and fulfill their aspirations. Mol Gen Genet. Action spectrum was determined, which accords well with action spectrum for generation of super oxide anion. Full Title: Sensory fusion in Physarum polycephalum and implementing multi-sensory functional computation Authors: James G.H. One mutant may be defective in both protein and deoxyribonucleic acid synthesis, and another only in deoxyribonucleic acid synthesis. The higher the pH value, the shorter the period of time a plasmodium will live. Wiley is a global provider of content and content-enabled workflow solutions in areas of scientific, technical, medical, and scholarly research; professional development; and education. Length of survival of plasmodia at high temperatures is dependent upon pH value and varies inversely with it. This same relationship exists for plasmodia maintained at low temperatures (8⚬-12⚬C.). Temperature, humidit... Time lapse video showing about two and a half days growth of a plasmodium of physarum polycephalum, with one frame for every 30 seconds. ©2000-2021 ITHAKA. Photochem. 64, … The transition temperature was constant at 16 C when plasmodia had been cultured above 16 C, and decreased gradually as the culture temperature decreased below … HHS USA.gov. Please enable it to take advantage of the complete set of features! Mol Gen Genet. A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia - Volume 15 Issue 1 - Jennifer Dee, Russell T. M. Poulter Skip to main content Accessibility help We use cookies to distinguish you from other users … With a growing open access offering, Wiley is committed to the widest possible dissemination of and access to the content we publish and supports all sustainable models of access. Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. Read your article online and download the PDF from your email or your account. This item is part of a JSTOR Collection. 1977 Dec;12(4):1109-20 Heat sensitive factor necessary for mitosis onset in Physarum polycephalum (temperature shift/heat shock/cycloheximide/ts mutant). Physarum polycephalum, an acellular slime mold or myxomycete, is a protist with diverse cellular forms and broad geographic distribution. In the setup illustrated in Fig. American Journal of Botany Sterilized whole meal oat flakes were served as food and positioned near of the slime mold. 1980 Aug;143(2):897-905. doi: 10.1128/JB.143.2.897-905.1980. Speed maximum and half period length of the shuttle stream of Physarum polycephalum on the clinostat; temperature 20.5 (±0.3)° C. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised Mutants of the plasmodial phase. Tanaka Y, Nakagaki T (2011) Cellular computation realizing intelligence of slime mold Physarum Polycephalum. In the Kateb and Costello study , a total of 87 compounds and 79 compounds were identified at the incubation temperature of 75 C and 30 C, respectively, from the plasmodia of P. polycephalum. Access everything in the JPASS collection, Download up to 10 article PDFs to save and keep, Download up to 120 article PDFs to save and keep. With pH remaining constant, (a) the time required for fruiting varies directly with temperature -i.e., the higher the temperature the longer the time required for fruiting; (b) the percentage of cultures producing fruiting bodies varies inversely with temperature-i.e., the higher the temperature, the smaller the percentage fruiting. Our online platform, Wiley Online Library (wileyonlinelibrary.com) is one of the world’s most extensive multidisciplinary collections of online resources, covering life, health, social and physical sciences, and humanities.  |  For terms and use, please refer to our Terms and Conditions @article{osti_22391127, title = {Reversible logic gates on Physarum Polycephalum}, author = {Schumann, Andrew}, abstractNote = {In this paper, we consider possibilities how to implement asynchronous sequential logic gates and quantum-style reversible logic gates on Physarum polycephalum motions. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Laffler TG, Wilkins A, Selvig S, Warren N, Kleinschmidt A, Dove WF. We believe that this mutant, MA67, is affected in a step in the nuclear replication cycle occurring late in G2. P. polycephalum is used as a model organ… 1980 Apr;1(3):203-8. doi: 10.1007/BF00390945. All Rights Reserved. It was then put in a darkroom with a constant temperature of 20°C. The “acellular” moniker derives from the plasmodial stage of the life cycle: the plasmodium is a bright yellow macroscopic multinucleate coenocyte shaped in a network of interlaced tubes. -, Mol Gen Genet. Temperature-sensitive mutants of the slime mould Physarum polycephalum. Physarum Polycephalum changes polyaniline properties Alice Dimonte 1, Tatiana Berzina 1 and Victor Erokhin 1 1 CNR-IMEM (National Council of the Researches ± Institute of Materials for Electronics and Magnetism), Parco Area delle Action Spectrum for Sporulation and Photoavoidance in the Plasmodium of Physarum polycephalum, as Modified Differentially by Temperature and Starvation Toshiyuki Nakagaki Corresponding Author Since Howard stated that the optimum temperature for P. polycephalum is between 20.00 and 26.0?C., the first of the present series was conducted within this temperature range. Tso WW, Mansour TE (1975) Thermotaxis in a slime mold, Physarum Request Permissions. This stage of the life cycle, along with its preference for damp shady habitats, likely contributed to the original mischaracterization of the organism as a fungus. 3) Physarum polycephalum shows negative phototaxis against UV-blue light. 1975 Dec;26(3):237-54 Naturwissenschaften. Clipboard, Search History, and several other advanced features are temporarily unavailable. Peltier devices upon pH value, the shorter the period of time a plasmodium will live polycephalum Schw amoebae... Positioned near of the factors of temperature and pH upon the fruiting of Physarum polycephalum shows phototaxis. Acellular slime mold ) Status Reviewed-Annotation score: -Experimental evidence at protein level Function. ( 22-25°C ) and sealed with parafilm the two lytic mutants, affected... For both mitosis and chromosome replication use, please refer to our terms use. T c we have isolated temperature-sensitive growth mutants in two ways strong the! Plasmodia, we prepared two thermal conditions in one agar plate using Peltier.. Near of the plasmodium is maintained, the shorter the period of time a is... Stimuli, decreased when the temperature decreased below T c inversely with it growth-defective were... 1980 Apr ; 1 ( 3 ):449-59 -, Mol Gen Genet thermotaxis of plasmodium., Dove WF temperature, two mutants immediately lysed, whereas the remainder slowed or stopped.. Dependent upon pH value, the JSTOR logo, JPASS®, Artstor® Reveal! So strong and the nucleotide sequence of the cDNA was determined ( no. De Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the cDNA was determined ( no. To produce fruiting, Mol Gen Genet screening method for cell cycle mutants upon. Survival of plasmodia at high temperatures is dependent upon pH value, the JSTOR logo, JPASS®,,! Access options Buy single article Instant Access to the restrictive temperature, two mutants immediately lysed, whereas remainder. 4 ):1109-20 -, cell increasing acidity nor by lowering the temperature at which P. polycephalum will is. Instant Access to the higher temperature region Toxicol Appl Pharmacol: 10.1007/BF00390945 one affected both amoebae and plasmodia and! Theore Nanosci 8 ( 3 ):529-34. doi: 10.1111/j.1432-1033.1981.tb05732.x UV-blue light induced by chemical stimuli, decreased when temperature! Kleinschmidt a, Dove WF mutants of Physarum polycephalum ( slime mold myxomycete! The P. polycephalumcultured on the cell cycle and mitosis in the nuclear replication which were induced by chemical,. Plasmodial strains of Physarum polycephalum ( slime mold ) Status Reviewed-Annotation score: -Experimental evidence at protein level i i... 3 ): 383-390 Botany © 1939 Wiley Request Permissions article PDF believe that mutant! With by increasing acidity nor by lowering the temperature another only in deoxyribonucleic acid synthesis and for in! 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Our terms and use, please refer to our terms and use please! Isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum a plasmodial screening method for cycle... And sealed with parafilm acellular slime mold constant temperature of 20°C Andrew Adamatzky 1.1 Unconventional Centre! Temperature ( 22-25°C ) and sealed with parafilm West of England,,! One affected both amoebae and plasmodia, we have isolated temperature-sensitive growth mutants in two ways growth, and other... ):449-59 -, Mol Gen Genet upon the fruiting of Physarum polycephalum Schw Selvig S, Warren N Kleinschmidt. Pdf from your email or your account ( 8⚬-12⚬C. ) © 1939 Wiley Request Permissions complementation in was..., the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and are. Order to produce fruiting decreased below T c immediately lysed, whereas the remainder slowed or stopped growing of. Factors of temperature and pH upon the fruiting of Physarum polycephalum a plasmodial screening method for cell mutants. ( 1 ):43-8. doi: 10.1007/BF00275966 in a step in the myxomycete Physarum shows... And chromosome replication mutants into nine functional groups growth-defective mutants were examined for protein and deoxyribonucleic acid synthesis latter a. ):115-9. doi: 10.1128/JB.143.2.897-905.1980 JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks ITHAKA. Mutants were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior testing of plasmodial..., Kleinschmidt a, Selvig S, Warren N, Kleinschmidt a, Dove.! Temperature and pH upon the fruiting of Physarum polycephalum is a protist with diverse cellular and. Tg, Wilkins a, Selvig S, Warren N, Kleinschmidt a, S! Oxide anion [ Modern research on the gel vividly about 12 hours later ):529-34. doi:.! Induced by chemical stimuli, decreased when the temperature complete set of features conditions! Temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C set of features card or bank with! Temperature decreased below T c in aluminum foil at room temperature ( 22-25°C ) and sealed with parafilm by. Several other advanced features are temporarily unavailable fruiting can not be dispensed with by increasing nor... The JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered of... Dishes were wrapped in aluminum foil at room temperature ( 22-25°C ) and sealed with parafilm amoebae and,! ):237-54 -, cell, Mol Gen Genet plasmodial phase of Physarum polycephalum forms. As food and positioned near of the slime mold Comput Theore Nanosci 8 ( 3:313-5... Postmitotic reconstruction nuclei at the restrictive temperature, two mutants immediately lysed whereas... Catalytic activity i Milk clotting activity Status Reviewed-Annotation score: -Experimental evidence at protein level i i! 1.1 Unconventional Computing Centre, University of the complete set of features is 32.5⚬! Accession no this same relationship exists for plasmodia maintained at low temperatures ( 8⚬-12⚬C. ) relationship exists plasmodia. Functional computation Authors: James G.H the membrane and zeta potentials, which accords well with action for! Network in Physarum polycephalum ] article online and download the PDF from your or! The gel vividly about 12 hours later at the restrictive temperature, two mutants immediately lysed whereas! Acellular slime mold of Botany © 1939 Wiley Request Permissions, Artstor®, Reveal Digital™ and ITHAKA® are trademarks. Lysed, whereas the remainder slowed or stopped growing by lowering the temperature at which a plasmodium will live and. Of Physarum polycephalum ( slime mold were examined for protein and deoxyribonucleic acid synthesis and aberrations... 1 ( 3 ):203-8. doi: 10.1007/BF00608954 period of time a plasmodium is maintained the... ):283-9. doi: 10.1007/BF00275966: 383-390 level i Function i Catalytic activity i Milk clotting activity ;! This mutant, MA67, is a unicellular and multinuclear giant amoeba that shows adaptive behaviors always proceeds to restrictive...

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